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Data from: A fast method for the detection of <i>Salmonella</i> Typhimurium cells in egg biofilms on three different surfaces

Metadata Updated: April 21, 2025

Salmonella is a pathogenic microorganism linked to foodborne outbreaks associated with egg and egg products. This microorganism can resist sanitation of the egg processing equipment and form biofilms. The main challenge is detecting Salmonella cells in the early stages of biofilm formation to use effective interventions to control and remove the Salmonella biofilms. This work aimed to study the biofilm formation of S. Typhimurium in liquid whole egg (LWE) on three common food-contact surfaces, stainless steel, silicone, and nylon, during the first five hours of incubation at 37°C and compare traditional microbiological methods to innovative and fast detection techniques. The results showed that using general plate counts, Salmonella cells were detected after three h of incubation with less than 1 – log of growth, silicone was the material with most cells attached, followed by stainless steel. Long-read whole genome sequencing detected Salmonella on stainless steel, silicone, and nylon after only one h of incubation. The results of this study suggest that long-read sequencing could be very useful for detecting Salmonella at low concentrations in the processing environment.

This research used the resources provided by SCINet project and the AI Center of Excellence of the USDA Agricultural Research Service, ARS project number 0500-00093-001-00-D.

Access & Use Information

Public: This dataset is intended for public access and use. License: us-pd

Downloads & Resources

Dates

Metadata Created Date November 2, 2024
Metadata Updated Date April 21, 2025

Metadata Source

Harvested from USDA JSON

Additional Metadata

Resource Type Dataset
Metadata Created Date November 2, 2024
Metadata Updated Date April 21, 2025
Publisher Agricultural Research Service
Maintainer
Identifier 10.15482/USDA.ADC/26866363.v1
Data Last Modified 2024-08-30
Public Access Level public
Bureau Code 005:18
Metadata Context https://project-open-data.cio.gov/v1.1/schema/catalog.jsonld
Schema Version https://project-open-data.cio.gov/v1.1/schema
Catalog Describedby https://project-open-data.cio.gov/v1.1/schema/catalog.json
Harvest Object Id eccd1c4f-0b34-42f2-9fbf-a7b6afca0845
Harvest Source Id d3fafa34-0cb9-48f1-ab1d-5b5fdc783806
Harvest Source Title USDA JSON
License https://www.usa.gov/publicdomain/label/1.0/
Program Code 005:040
Source Datajson Identifier True
Source Hash 9dfeddcaade1a7889300edb24fa0e5dbd6b4b5253540dbfc6a94f06f083c0938
Source Schema Version 1.1
Temporal 2024-01-31/2024-08-02

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