The web-based tool Fate and Transport of Indoor Microbiological Aerosols (FaTIMA) allows the determination of the fate of indoor microbiological aerosols associated with ventilation, filtration, deposition, and inactivation mechanisms. FaTIMA provides a representation of a single, well-mixed zone that is served by a mechanical ventilation system and incorporates source and removal mechanisms for an aerosol with a single, user-defined representative particle size. The simple mechanical ventilation system model allows specification of supply, return, and outdoor air intake rates to represent either a positive, negative or balanced ventilation system. Aerosol sources are provided to enable any combination of continuous, e.g., breathing-related emissions, or intermittent, e.g., coughing-related emissions. Aerosol removal mechanisms include filters within the ventilation system, room air cleaners, and deposition onto floors, walls, and ceilings. Simulations can be run for a 24-h period, with the results including the time history of the airborne concentration and surface loading, as well as integrated exposure of an occupant. Exposure is based on a uniform aerosol concentration within the zone and does not account for spatial differences in aerosol concentrations such as those due to momentum effects of respiratory emissions.