Microarray analysis techniques: Difference between revisions

Revision as of 17:35, 2 May 2012

Revision as of 06:15, 5 October 2012

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==Background correction==

Depending on the type of array, signal related to nonspecific binding of the fluorophore can be subtracted to achieve better results. One approach involves subtracting the average

signal intensity of the area between spots. A variety of tools for background correction and further analysis are available from TIGR,<ref>{{cite web |url=http://www.tigr.org/software/microarray.shtml |title=J. Craig Venter Institute -- Software |accessdate=2008-01-01 |work=}}</ref> Agilent ([[GeneSpring]]),<ref>{{cite web |url=http://www.chem.agilent.com/scripts/pds.asp?lpage=27881 |title=Agilent &#124; GeneSpring GX |accessdate=2008-01-02 |format= |work=}}</ref> and [[Ocimum BiosolutionsBio Solutions]] (Genowiz).<ref>{{cite web |url=http://wwwwww3.ocimumbio.com/webdata-analysis-insights/bioinformaticanalytical-tools/genowiz/prod_details.asp?prod_id=20&prodType=1 |title=Ocimum Biosolutions &#124; Genowiz |accessdate=2009-04-02 |format= |work=}}</ref>

==Quality control==