SSC buffer: Difference between revisions

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In [[biochemistry]] and [[molecular biology]], the '''saline-sodium citrate (SSC) buffer''' is used as a hybridisationhybridization [[buffer (chemistry)|buffer]], to control stringency for washing steps in protocols for [[Southern blotting]], [[in situ hybridization]], [[DNA Microarray]] or [[Northern blotting]]. 20X SSC may be used to prevent drying of [[agarose gel]]s during a vacuum transfer.

{{Orphan|date=February 2009}}

{{Notability|date=June 2008}}

{{context}}

TheA 20×20X stock solution consists of 3 [[molar (concentration)|M]] [[sodium chloride]] and 300 [[milli-|m]]M [[trisodium citrate]] (adjusted to [[pH]] 7.0 with [[hydrochloric acid|HCl]]).

In [[biochemistry]] and [[molecular biology]], the '''saline-sodium citrate (SSC) buffer''' is used as a hybridisation [[buffer (chemistry)|buffer]], to control stringency for washing steps in protocols for [[Southern blotting]], [[DNA Microarray]] or [[Northern blotting]].

The 20× stock solution consists of 3 [[molar (concentration)|M]] [[sodium chloride]] and 300 [[milli-|m]]M [[trisodium citrate]] (adjusted to [[pH]] 7.0 with [[hydrochloric acid|HCl]]).

==See also==

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*[[Northern Blot]]

*[[DNA Microarray]]

*[[DNA Fingerprinting]]

==References==

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* [http://www.le.ac.uk/bl/phh4/probuffer.htm Molecular Cytogenetics and biology Buffers, University of Leicester]

{{DEFAULTSORT:Ssc Buffer}}

[[Category:BuffersBuffer solutions]]