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Revision as of 11:05, 23 January 2011 edit Yobot (talk \| contribs) Bots 4,733,870 edits m WP:CHECKWIKI error fixes + general fixes, removed orphan tag using AWB (7567) ← Previous edit		Latest revision as of 22:18, 16 January 2022 edit undo JJMC89 bot III (talk \| contribs) Bots, Administrators 4,345,227 edits m Moving Category:Buffers to Category:Buffer solutions per Wikipedia:Categories for discussion/Speedy
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Line 1: In [[biochemistry]] and [[molecular biology]], ~~the~~ '''saline-sodium citrate (SSC) buffer''' is used as a hybridization [[buffer (chemistry)\|buffer]], to control stringency for washing steps in protocols for [[Southern blotting]], [[in situ hybridization]], [[DNA Microarray]] or [[Northern blotting]]. 20X SSC may be used to prevent drying of [[agarose gel]]s during a vacuum transfer. ▼ ~~{{Multiple issues\|notability =June 2008\|context =October 2009}}~~ ▲In [[biochemistry]] and [[molecular biology]], the '''saline-sodium citrate (SSC) buffer''' is used as a hybridization [[buffer (chemistry)\|buffer]], to control stringency for washing steps in protocols for [[Southern blotting]], [[in situ hybridization]], [[DNA Microarray]] or [[Northern blotting]]. 20X SSC may be used to prevent drying of [[agarose gel]]s during a vacuum transfer. A 20X stock solution consists of 3 [[molar (concentration)\|M]] [[sodium chloride]] and 300 [[milli-\|m]]M [[trisodium citrate]] (adjusted to [[pH]] 7.0 with [[hydrochloric acid\|HCl]]). Line 16 ⟶ 14: {{DEFAULTSORT:Ssc Buffer}} [[Category:~~Buffers~~Buffer solutions]]