Selector technique: Difference between revisions

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Line 3: == Process == Genomic [[DNA]] is digested with [[restriction enzymes]], circularized by [[hybridisation (molecular biology)\|hybridisation]] to selectors and subsequently attached to a [[Plasmid\|vector]] sequence by [[DNA ligase\|ligation]]. The procedure results in circular DNA [[molecules]] with an included general [[Primer (molecular biology)\|primer]] pair motif that can be used for amplification by [[Polymerase chain reaction\|PCR]] or [[Rolling circle replication\|RCA]]. ==Selector construct== Line 15: ==Publications== [https://web.archive.org/web/20051127101236/http://nar.oxfordjournals.org/cgi/content/full/33/8/e71 Demonstration of the selector method] [https://archive.today/20130415163620/http://nar.oxfordjournals.org/cgi/content/full/33/8/e72 The PieceMaker software for designing selector experiments] {{DEFAULTSORT:Selector-Technique}}