Synthetic genetic array: Difference between revisions

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==Procedure==

Synthetic genetic array analysis is generally conducted using colony arrays on petriplates at standard densities (96, 384, 768, 1536). To perform a SGA analysis in ''S.cerevisaecerevisiae'', the query gene deletion is crossed systematically with a deletion mutant array (DMA) containing every viable knockout [[Open reading frame|ORF]] of the yeast genome (currently 4786 strains).<ref>{{cite web|title=Yeast Knockout Strains

|url=http://www.openbiosystems.com/GeneExpression/Yeast/YKO/|archiveurl=https://web.archive.org/web/20111119012937/http://www.openbiosystems.com/GeneExpression/Yeast/YKO/|archivedate=November 19, 2011 |work=Open Biosystems}}</ref> The resulting [[diploids]] are then sporulated by transferring to a media containing reduced nitrogen. The [[haploid]] progeny are then put through a series of selection platings and incubations to select for double mutants. The double mutants are screened for SSL interactions visually or using imaging software by assessing the size of the resulting colonies.

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Due to the large number of precise replication steps in SGA analysis, robots are widely used to perform the colony manipulations. There are a few systems specifically designed for SGA analysis, which greatly decrease the time to analyse a query gene. Generally these have a series of pins which are used to transfer cells to and from plates, with one system utilizing disposable pads of pins to eliminate washing cycles. Computer programs can be used to analyze the colony sizes from images of the plates thus automating the SGA scoring and chemical-genetics profiling.

== StepSteps for a yeast high content genome-wide genetic screening system (SGA -road map) ==

There are six major components:

# Mutant collection

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# Confirmation approaches

# Data analysis tools

* Mutant collection

The first step is to collect the mutants and create a mutants library either in solid or liquid media. Solid media could be better because it could save lots of time. At Early stage, mutant creation was done by homologous recombination method. We have an excellent mutant library for [[Saccharomyces cerevisiae]], a well-studied model organism.

However, if you are trying for new, yeast model, you could have to either a genome sequencing and can predict the possible ORF by the good reference yeast genome(For example: with Saccharomyces cerevisiae). Consider a special case: If you don't have a reference genome, you should go for [[Transcriptomics technologies|transcriptome]] and genome analysis of that new model organism.

* Material and tools for handling the mutants<br />Once you have your mutant library in solid media. If mutants are in solid media, we have arranged the mutants with 1:3 ration, i.e. for one wild type to 3 mutants array(why? Wild type works as an internal control and in a solid media nutrient should not be shared equally for avoiding bias). Once you have single gene deleted mutants, you can start the tools for handling the mutants. In SGA it is referred to as “ Pinning”. ROTOR-HAD versions(referred as pinning robot) used for pinning the yeast mutants. This machine installed with a user-friendly interface which helps to pin the samples from sources plates to experimental plates

#Image analysis system

# Automatic quantification and scoring system

# Confirmation approaches

# Data analysis tools

=== Mutant collection ===

==See also==