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==Untitled==
This article is lacking moleular level details in describing riflips. more examples and relevant ideas should be given. it's a good start but much could be added.
▲{{oldpeerreview|archive=1}}
from wikien-l
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:::I think that's awesome! Much clearer and nicer flowing than what I wrote. I've put it into the article. (I changed "base" to "nucleotide", since I think that's more specific -- change back if you disagree.) I wonder what Dr. X will think... [[User:Zashaw|Zashaw]] 02:30, 29 Apr 2004 (UTC)
Most commonly either RFLP or PCR is performed, not typically both. [[Special:Contributions/165.176.123.2|165.176.123.2]] ([[User talk:165.176.123.2|talk]]) 17:20, 23 January 2008 (UTC)
:::I believe that it may be "RFLP analysis" which many are thinking about. RFLP is just the variation that occurs. It's the analysis of that which is what I think many people are thinking of. I think "analysis" has just been dropped in regard to RFLP due to convienence. See that section below from: Web Database of Molecular Genetic Data From Fish Stocks, J Hered (2003) 94 (3): 265-267.
"The development of DNA amplification using the polymerase chain reaction (PCR) (Saiki et al. 1988) resulted in an increasing number of molecular techniques which are now being applied, such as RFLP analysis. Restriction enzymes are used to digest DNA strands at a specific ___location (recognition site), resulting in a number of DNA fragments. These fragments can be separated by gel electrophoresis, and differences in the pattern of the fragments between individuals are called RFLPs (Dowling et al. 1990). An RFLP may result from a base substitution that causes the gain or loss of a restriction site, or from an insertion/deletion mutation. " <span style="font-size: smaller;" class="autosigned">— Preceding [[Wikipedia:Signatures|unsigned]] comment added by [[Special:Contributions/137.149.98.24|137.149.98.24]] ([[User talk:137.149.98.24|talk]]) 21:26, 9 June 2011 (UTC)</span><!-- Template:UnsignedIP --> <!--Autosigned by SineBot-->
::::I do believe it is quite common to perform PCR before doing digests. If you only did the digests, you would have very little DNA to see in the agarose gel. For the work I perform, we only perform digests of the the 16S rDNA after PCR. Generally you want more product before you want to destroy what you have for the analysis.[[Special:Contributions/137.149.98.24|137.149.98.24]] ([[User talk:137.149.98.24|talk]]) 21:32, 9 June 2011 (UTC)
== the relation to vntr??? ==
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:You are right in thinking the two terms are related: in fact, VNTRs were often detected as RFLPs by Southern hybridization, though now more usually a PCR technique is used. The term VNTR refers to the variable sequence rather than to the method used to detect it. - [[User:Nunh-huh|Nunh-huh]] 19:59, 30 May 2006 (UTC)
== Discovery ==
Botstein, White, Skolnic, Davis (1980) Am. J Hum. Genet. 32(3) 314 defined RFLPs before the 1985 Jeffries citation listed currently <small><span class="autosigned">—Preceding [[Wikipedia:Signatures|unsigned]] comment added by [[User:GCdeQR|GCdeQR]] ([[User talk:GCdeQR|talk]] • [[Special:Contributions/GCdeQR|contribs]]) 06:46, 15 May 2009 (UTC)</span></small><!-- Template:Unsigned --> <!--Autosigned by SineBot-->
:Any idea if that's the first mention of it? Can we attribute its first use as a full assay to Botstein ''et al.'' in 1980? [[User:Qwerty0|Qwerty0]] ([[User talk:Qwerty0|talk]]) 15:57, 20 September 2018 (UTC)
::Oops, sorry, Botstein ''et al.'' didn't invent RFLP; they just referred to it. However, ''they'' claim that [http://symposium.cshlp.org/content/39/439 Grodzicker ''et al.'' 1974] did. [[User:Qwerty0|Qwerty0]] ([[User talk:Qwerty0|talk]]) 16:58, 20 September 2018 (UTC)
== Restriction Enzyme Site link ==
couldn't the red link in
: ... It refers to a [[polymorphism (biology)|difference]] between samples of [[homologous]] [[DNA]] molecules that come from differing locations of [[restriction enzyme sites]], and to a related laboratory technique ...
actually link to [[restriction site]]?
[[Special:Contributions/212.126.224.100|212.126.224.100]] ([[User talk:212.126.224.100|talk]]) 17:47, 21 February 2011 (UTC)
:Seems like a good place for it to link to, so now it does. Thanks for pointing that out. - <span style="font-family: cursive">[[User:Nunh-huh|Nunh-huh]]</span> 21:59, 21 February 2011 (UTC)
== History largely missing ==
Like a lot of technical articles on ''Wikipedia'', this one is spectacularly weak on history. Not just who discovered it, but when it became widely used, and when it became obsolescent. The science end of the article is great; the social end is largely absent. If anyone can supply just a few sentences charting its discovery, emergence, expanded use, and then obsolescence, that would be great. [[User:Theonemacduff|Theonemacduff]] ([[User talk:Theonemacduff|talk]]) 00:17, 4 November 2019 (UTC)
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