Random amplification of polymorphic DNA: Difference between revisions

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Revision as of 08:17, 12 May 2022 edit 103.29.209.63 (talk) No edit summary Tags: Mobile edit Mobile web edit ← Previous edit		Latest revision as of 11:44, 24 June 2025 edit undo Rbrwr (talk \| contribs) Administrators 13,518 edits Adding local short description: "Polymerase chain reaction technique", overriding Wikidata description "PCR technique used to generate strain-specific arrays of anonymous DNA fragments" Tag: Shortdesc helper
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Line 1: {{Short description\|Polymerase chain reaction technique}} [[File:RAPD.JPG\|thumb]] {{Refimprove\|date=June 2016}} '''Random ~~amplification of~~amplified polymorphic DNA''' ('''RAPD'''), pronounced "rapid",<ref>{{Cite web\|title=Random Amplified Polymorphic DNA (RAPD)\|url=https://www.ncbi.nlm.nih.gov/probe/docs/techrapd/\|access-date=2020-11-10\|website=www.ncbi.nlm.nih.gov}}</ref> is a type of [[polymerase chain reaction]] (PCR), but the segments of DNA that are amplified are random.<ref>{{Cite web\|title=rDNA: Random Amplification of Polymorphic DNA (RAPD)\|url=http://www.rvc.ac.uk/review/dna_1/5_RAPD.cfm\|access-date=2016-06-03\|website=www.rvc.ac.uk\|archive-date=2020-04-07\|archive-url=https://web.archive.org/web/20200407160724/http://www.rvc.ac.uk/review/DNA_1/5_RAPD.cfm\|url-status=dead}}</ref> The scientist performing RAPD creates several arbitrary, short primers (~~10- 12~~10–12 nucleotides), then proceeds with the PCR using a large template of genomic DNA, hoping that fragments will amplify. By resolving the resulting patterns, a semi-unique profile can be gleaned from an RAPD reaction.