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Random amplification of polymorphic DNA: Difference between revisions

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Adding local short description: "Polymerase chain reaction technique", overriding Wikidata description "PCR technique used to generate strain-specific arrays of anonymous DNA fragments"
 
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{{Short description|Polymerase chain reaction technique}}
[[File:RAPD.JPG|thumb]]
 
{{Refimprove|date=June 2016}}
'''Random amplification ofamplified polymorphic DNA''' ('''RAPD'''), pronounced "rapid",<ref>{{Cite web|title=Random Amplified Polymorphic DNA (RAPD)|url=https://www.ncbi.nlm.nih.gov/probe/docs/techrapd/|access-date=2020-11-10|website=www.ncbi.nlm.nih.gov}}</ref> is a type of [[polymerase chain reaction]] (PCR), but the segments of DNA that are amplified are random.<ref>{{Cite web|title=rDNA: Random Amplification of Polymorphic DNA (RAPD)|url=http://www.rvc.ac.uk/review/dna_1/5_RAPD.cfm|access-date=2016-06-03|website=www.rvc.ac.uk|archive-date=2020-04-07|archive-url=https://web.archive.org/web/20200407160724/http://www.rvc.ac.uk/review/DNA_1/5_RAPD.cfm|url-status=dead}}</ref> The scientist performing RAPD creates several arbitrary, short primers (10- 1210–12
nucleotides), then proceeds with the PCR using a large template of genomic DNA, hoping that fragments will amplify. By resolving the resulting patterns, a semi-unique profile can be gleaned from an RAPD reaction.
 
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