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'''Alsever's solution''' is a [[salinity|saline]] liquid used to prevent coagulation of blood.<ref>Alsever, J. B., & Ainslie, R. B. (1941). A new method for the preparation of dilute blood plasma and the operation of a complete transfusion service. NY State J. Med, 41, 126-131.</ref><ref>{{cite book|last1=Campbell|first1=D. H.|title=Methods in Immunology. A Laboratory Text for Instruction and Research|date=1963|publisher=W. A. Benjamin, Inc.|___location=New York|page=244|last2=Garvey |first2=J. S. |last3=Cremer |first3=N. E. |last4=Sussdorf |first4=D. H.}}</ref> It is composed of 2.05% [[dextrose]], 0.8% [[sodium citrate]], 0.055% [[citric acid]], and 0.42% [[sodium chloride]]. For usage, an equal volume of blood is gently, but thoroughly, mixed with the solution. This solution is used to study ''[[in vitro]]'' anti-inflammatory activity of crude drugs by the [[human red blood cell stabilization]] method.<ref>Gandhisan R, Thamaraichelvan A, Baburaj. Antiinflammatory action of Lannea coromandelica HRBC membrane stabilization. Fitotherapia 1991; 62: 82-83.</ref> It is also used to preserve blood cells from other sources.<ref>{{cite journal | doi = 10.1111/j.1699-0463.1953.tb00242.x| title = Experiments on the Stability of Sheep Erythrocytes Stored in Alsever's Solution| journal = Acta Pathologica et Microbiologica Scandinavica| volume = 32| pages = 198–203| year = 2009| last1 = Schjerning-Thiesen| first1 = Knud| issue = 1| pmid = 13050415}}</ref><ref>{{cite book| url= https://books.google.com/books?id=OLd91TkawxMC&pg=PA827 | page=827| title=L.S.A., List of C.F.R. Sections Affected| year=2003}}</ref>
The test was invented in 1941 by the American Hematologist, John Bellows Alsever (1908–1978).<ref>{{Cite web|url=https://medical-dictionary.thefreedictionary.com/John+Bellows+Alsever|title=John Bellows Alsever|website=TheFreeDictionary.com}}</ref>
==References==
{{Reflist}}
[[Category:Solutions]]
[[Category:Cell culture reagents]]
[[Category:Biochemistry methods]]
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