Content deleted Content added
Citation bot (talk | contribs) Add: year. | Use this bot. Report bugs. | Suggested by AwfulReader | Category:Molecular biology | #UCB_Category 271/686 |
Citation bot (talk | contribs) Removed URL that duplicated identifier. | Use this bot. Report bugs. | #UCB_CommandLine |
||
| (2 intermediate revisions by 2 users not shown) | |||
Line 1:
{{Multiple issues|
{{more footnotes|date=May 2014}}
{{Technical|date=July 2022}}
}}
The '''nonribosomal code''' refers to key amino acid residues and their positions within the primary sequence of an adenylation ___domain of a [[nonribosomal peptide synthetase]] used to predict substrate specificity and thus (partially) the final product. Analogous to the nonribosomal code is prediction of peptide composition by DNA/RNA codon reading, which is well supported by the [[central dogma of molecular biology]] and accomplished using the genetic code simply by following the [[DNA codon table]] or [[RNA codon table]]. However, prediction of natural product/secondary metabolites by the nonribosomal code is not as concrete as DNA/RNA codon-to-amino acid and much research is still needed to have a broad-use code. The increasing number of sequenced genomes and high-throughput prediction software has allowed for better elucidation of predicted substrate specificity and thus natural products/secondary metabolites. Enzyme characterization by, for example, ATP-pyrophosphate exchange assays for substrate specificity, ''[[in silico]]'' substrate-binding pocket modelling and structure-function mutagenesis (''in vitro'' tests or ''in silico'' modelling) helps support predictive algorithms. Much research has been done on bacteria and fungi, with prokaryotic bacteria having easier-to-predict products.
Line 9 ⟶ 8:
==Supporting examples==
The A-domains have 8 amino acid-long non-ribosomal signatures.<ref>{{Cite book|last=Compeau, Phillip
'''LTKVGHIG''' → Asp (Aspartic acid)
| |||