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{{Short description|Mathematical function in general imaging}}
[[File:Contrast transfer function.jpg|thumb|Power spectrum (Fourier transform) of a typical electron micrograph. The effect of the contrast transfer function can be seen in the alternating light and dark rings (Thon rings), which show the relation between contrast and spatial frequency. ]]
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==Phase contrast in HRTEM==
The contrast in HRTEM comes from interference in the image plane between the phases of scattered [[electron]] waves with the phase of the transmitted electron wave.
Detectors are only able to
▲The contrast in HRTEM comes from interference in the image plane between the phases of scattered [[electron]] waves with the phase of the transmitted electron wave. When an electron wave passes through a sample in the TEM, complex interactions occur. Above the sample, the electron wave can be approximated as a plane wave. As the electron wave, or [[wavefunction]], passes through the sample, both the [[phase (waves)|phase]] and the [[amplitude]] of the electron beam is altered. The resultant scattered and transmitted electron beam is then focused by an objective lens, and imaged by a detector in the image plane.
▲Detectors are only able to directly measure the amplitude, not the phase. However, with the correct microscope parameters, the [[Interference (wave propagation)|phase interference]] can be indirectly measured via the intensity in the image plane. Electrons interact very strongly with [[crystalline]] solids. As a result, the phase changes due to very small features, down to the atomic scale, can be recorded via HRTEM.
== Contrast transfer theory ==
[[File:TEM Ray Diagram with Phase Contrast Transfer Function.pdf|thumb|TEM Ray Diagram with Phase Contrast Transfer Function]]
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==Mathematical form==
If we incorporate some assumptions about our sample, then an analytical expression can be found for both phase contrast and the phase contrast transfer function. As discussed earlier, when the electron wave passes through a sample, the electron beam interacts with the sample via scattering, and experiences a phase shift. This is represented by the electron wavefunction exiting from the bottom of the sample. This expression assumes that the scattering causes a phase shift (and no amplitude shift). This is called the ''Phase Object Approximation.''
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=== The phase contrast transfer function ===
Passing through the objective lens incurs a Fourier transform and phase shift. As such, the wavefunction on the back focal plane of the objective lens can be represented by:<br />
:<math>I(\theta) = \delta(\theta) + \Phi K(\theta)</math>
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<math>\lambda</math> = the relativistic wavelength of the electron wave, <math>C_s</math> = The [[spherical aberration]] of the objective lens
<br />The contrast transfer function can also be given in terms of spatial frequencies, or reciprocal space. With the relationship <math display="inline">\theta =\lambda k</math>, the phase contrast transfer function becomes: ▼
▲The contrast transfer function can also be given in terms of spatial frequencies, or reciprocal space. With the relationship <math display="inline">\theta =\lambda k</math>, the phase contrast transfer function becomes:
:<math>K(k) = \sin[(2\pi) W(k)]</math><br /> <math>W(k) = -z\lambda k^2/2 + C_s\lambda^3 k^4/4</math>
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== Examples ==
The contrast transfer function determines how much phase signal gets transmitted to the real space wavefunction in the image plane. As the [[modulus squared]] of the real space wavefunction gives the image signal, the contrast transfer function limits how much information can ultimately be translated into an image. The form of the contrast transfer function determines the quality of real space image formation in the TEM.
[[File:Unmodified CTF.pdf|thumb|CTF Function prepared via web applet created by Jiang and Chiu, available at http://jiang.bio.purdue.edu/software/ctf/ctfapplet.html]]▼
▲[[File:Unmodified CTF.pdf|thumb|CTF Function prepared via web applet created by Jiang and Chiu, available at
This is an example contrast transfer function. There are a number of things to note:
* The function exists in the spatial frequency ___domain, or k-space
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=== Scherzer defocus ===
The defocus value (<math display="inline">z</math>) can be used to counteract the spherical aberration to allow for greater phase contrast. This analysis was developed by Scherzer, and is called the Scherzer defocus.<ref>{{Cite journal| doi = 10.1063/1.1698233 |title = The theoretical resolution limit of the electron microscope|last = Scherzer|date = 1949|journal = Journal of Applied Physics|volume=20 |issue = 1|pages=20–29|bibcode = 1949JAP....20...20S }}</ref>
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[[File:CTF Modified by Spatial and Temporal Envelope Functions.pdf|thumb|CTF Function of a CM300 Microscope damped by temporal and spatial envelope functions.]]
The envelope function represents the effect of additional aberrations that damp the contrast transfer function, and in turn the phase. The envelope terms comprising the envelope function tend to suppress high spatial frequencies. The exact form of the envelope functions can differ from source to source. Generally, they are applied by multiplying the Contrast Transfer Function by an envelope term Et representing temporal aberrations, and an envelope term Es representing spatial aberrations. This yields a modified, or effective Contrast Transfer Function:
<math>K_{eff}(k) = E_tE_s(\sin[(2\pi/\lambda)W(k)]</math>
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Examples of temporal aberrations include chromatic aberrations, energy spread, focal spread, instabilities in the high voltage source, and instabilities in the objective lens current. An example of a spatial aberration includes the finite incident beam convergence.<ref>{{Cite web|title = Envelope Functions|url = http://www.maxsidorov.com/ctfexplorer/webhelp/envelope_functions.htm|website = www.maxsidorov.com|access-date = 2015-06-12}}</ref>
<br />As shown in the figure, the most restrictive envelope term will dominate in damping the contrast transfer function. In this particular example, the temporal envelope term is the most restrictive. Because the envelope terms damp more strongly at higher spatial frequencies, there comes a point where no more phase signal can pass through. This is called the ''Information Limit'' of the microscope, and is one measure of the resolution.▼
▲As shown in the figure, the most restrictive envelope term will dominate in damping the contrast transfer function. In this particular example, the temporal envelope term is the most restrictive. Because the envelope terms damp more strongly at higher spatial frequencies, there comes a point where no more phase signal can pass through. This is called the ''Information Limit'' of the microscope, and is one measure of the resolution.
<br /> Modeling the envelope function can give insight into both TEM instrument design, and imaging parameters. By modeling the different aberrations via envelope terms, it is possible to see which aberrations are most limiting the phase signal.
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== Linear imaging theory vs. non-linear imaging theory ==
The previous description of the contrast transfer function depends on ''linear imaging theory''. Linear imaging theory assumes that the transmitted beam is dominant, there is only weak phase shift by the sample. In many cases, this precondition is not
▲The previous description of the contrast transfer function depends on ''linear imaging theory''. Linear imaging theory assumes that the transmitted beam is dominant, there is only weak phase shift by the sample. In many cases, this precondition is not fulilled. In order to account for these effects, ''non-linear imaging theory'' is required. With strongly scattering samples, diffracted electrons will not only interfere with the transmitted beam, but will also interfere with each other. This will produce second order diffraction intensities. Non-linear imaging theory is required to model these additional interference effects.<ref>{{Cite journal|title = Contrast Transfer Theory for Non-Linear Imaging|last = Bonevich, Marks|date = May 24, 1988|journal = Ultramicroscopy|doi = 10.1016/0304-3991(88)90230-6|volume=26|issue = 3|pages=313–319}}</ref><ref>This page was prepared in part for Northwestern University class MSE 465, taught by Professor Laurie Marks.</ref>
Contrary to a widespread assumption, the linear/nonlinear imaging theory has nothing to do with [[Diffraction formalism|kinematical diffraction]] or [[Dynamical theory of diffraction|dynamical diffraction]], respectively.
Linear imaging theory is still used, however, because it has some computational advantages. In Linear imaging theory, the Fourier coefficients for the image plane wavefunction are separable. This greatly reduces computational complexity, allowing for faster computer simulations of HRTEM images.<ref>[http://www.numis.northwestern.edu/465/index.shtml Notes] prepared by Professor [[Laurence D. Marks|Laurie Marks]] at Northwestern University.</ref>
== See also ==
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* [http://em-outreach.ucsd.edu/web-course/ref2.html CTF reading list]
* [http://www.maxsidorov.com/ctfexplorer/index.htm Interactive CTF Modeling]
{{Electron microscopy}}
[[Category:Microscopes]]
[[Category:Protein structure]]
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