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''This is a list of retired rikishi who have "completed" articles. They are complete in the sense that they have infoboxes, tournament tables, and biographical/career information that is more than a few lines. They could always use YOUR additions and improvements of course!''
[[Image:TaqPol.jpg|thumb|Structure of Taq polymerase]]
'''Taq polymerase''' ("Taq Pol," or simply "Taq", from "Thermus aquaticus") is a thermostable [[polymerase]] used [[PCR|polymerase chain reaction]] to check for the presence or absence of a gene by creating a DNA fragment. First isolated from ''[[Thermus aquaticus]]'', a [[bacterium]] that lives in [[hot springs]] and [[hydrothermal vent]]s, Taq was identified as the first polymerase able to withstand the denaturing conditions required during PCR. Its enzymatic activity [[halflife]] at 95 degrees [[Celsius]] is 1.6 hours.
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One of Taq polymerases major drawbacks is its low replication [[fidelity]]. Without a 3' to 5' [[exonuclease]] [[proofreading]] capacity to replace an accidental mismatch in the newly [[Chemical synthesis|synthesized]] [[DNA]] strand, Taq polymerase cannot be used in experiments where an identical genetic sequence is required, such as in molecular cloning. Commerically sold Taq DNA polymerase has an error rate of one in 10,000 nucleotides and typically produces 16% of mutated 1kb (kilobasepair) PCR products in a reaction. It can amplify a 1kb strand of DNA in roughly 30 seconds at 72°C.
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Taq DNA polymerase also yields fragments with A (Adenine) overhangs. This is particularly useful in 'TA Cloning,' whereby a cloning vector (such as a plasmid) is used which has T (Thymine) overhangs which complement with the A overhangs of the PCR Product, thus increasing the ligation efficiency.
;A - O
*[[Akebono]]
[[Pfu DNA polymerase]] is often used instead of, or in conjunction with, Taq polymerase. This enzyme is more thermostable and proofreads DNA, resulting in a lower error rate.
*[[Akinoshima]]
*[[Asahifuji]]
== See also ==
*[[Chiyonofuji]]
*[[Futahaguro]]
[[Pfu DNA polymerase]]
*[[Hokutoumi]] column style tourney table
*[[Kitanofuji]]
{{Commons|Taq polymerase}}
*[[Kitanoumi]]
*[[Konishiki]]
[[category:microbiology]]
*[[Kotonishiki]]
[[category:DNA replication]]
*[[Kotonowaka]]
[[Category:EC 2.7.7]]
*[[Kotozakura]]
Taq DNA polymerase replaced E.Coli DNA Polymerase because of the tempature conditions of PCR.
*[[Kyokushuzan]] bio a little short
*[[Musashimaru]]
[[de:Taq-Polymerase]]
*[[Onokuni]]
[[sv:Taq polymerase]]
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;R - Z
*[[Ryūko]]
*[[Sakahoko]]
*[[Sentoryu]]
*[[Taiho]]
*[[Takamiyama]]
*[[Takanohana II]]
*[[Takanonami]]
*[[Takatoriki]]
*[[Tamanoumi]]
*[[Terao]]
*[[Tochiazuma]]
*[[Toki Susumu]]
*[[Wajima Hiroshi|Wajima]]
*[[Wakanohana Kanji II|Wakanohana]] II
*[[Wakanohana]] III
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