Human Protein Reference Database: Difference between revisions

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{{Short description|Online database of proteins}}

{{primarysourcesprimary sources|date=November 2008}}

[[Image:HPRD.JPG|thumb|right|300 px|The HPRD provides a comprehensive link to any queried protein]]

The '''Human Protein Reference Database'''(HPRD) is a protein database accessible through the internet.<ref>Peri, S. ''et al.'' Development of human protein reference database as an initial platform for approaching systems biology in humans. ''Genome Research''. 2003. 13, 2363-2371</ref>

The '''Human Protein Reference Database''' ('''HPRD''') is a [[protein]] [[database]] accessible through the [[Internet]].<ref>{{Cite journal |vauthors=Peri S, etal |title=Development of human protein reference database as an initial platform for approaching systems biology in humans |journal=Genome Research |year=2003 |volume=13 |issue=10 |pages=2363–71 |doi=10.1101/gr.1680803 |pmid=14525934 |pmc=403728}}</ref> It is closely associated with the premier [[India|Indian]] Non-Profit research organisation [[Institute of Bioinformatics, Bangalore| Institute of Bioinformatics]] (IOB), [[Bangalore]], [[India]]. This database is a collaborative output of IOB and the Pandey Lab of [[Johns Hopkins University]].

The HPRD is a result of an international collaborative effort between the [http://www.ibioinformatics.org/ Institute of Bioinformatics] in Bangalore, India and the [http://pandeylab.igm.jhmi.edu/ Pandey lab] at [[Johns Hopkins University]] in Baltimore, USA. HPRD contains manually curated scientific information pertaining to the biology of most human proteins. Information regarding proteins involved in human diseases is annotated and linked to [http://www.ncbi.nlm.nih.gov/sites/entrez?db=omim Online Mendelian Inhertance in Man] (OMIM) database. The [http://www.ncbi.nlm.nih.gov/ National Center for Biotechnology Information] provides link to HPRD through its human protein databases (e.g. [http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene Entrez Gene], [http://www.ncbi.nlm.nih.gov/RefSeq/ RefSeq protein]) pertaining to genes and proteins.

==Overview==

This resource depicts information on human protein functions including [[protein-protein interactions]], [[post-translational modifications]], enzyme-substrate relationships and [[disease]] associations. Protein annotation information that is catalogued was derived through manual curation using published literature by expert biologists and through bioinformatics analyses of the protein sequence. The protein-protein interaction and subcellular localization data from HPRD have been used to develop a human protein interaction network.<ref>Gandhi, T.K.B. ''et al.'' Analysis of the human protein interactome and comparison with yeast, worm and fly interaction datasets. ''Nature Genetics''. 2006. 3, 285-293</ref>

The HPRD is a result of an international collaborative effort between the [http://www.ibioinformatics.org/[Institute of Bioinformatics, Bangalore| Institute of Bioinformatics]] in Bangalore, India and the [http://pandeylab.igm.jhmi.edu/[Akhilesh Pandey (scientist)| Pandey lab]] at [[Johns Hopkins University]] in Baltimore, USA. HPRD contains manually curated scientific information pertaining to the biology of most human proteins. Information regarding proteins involved in human diseases is annotated and linked to [http://www.ncbi.nlm.nih.gov/sites/entrez?db=omim Online Mendelian InhertanceInheritance in Man] (OMIM) database. The [http://www.ncbi.nlm.nih.gov/ National Center for Biotechnology Information] provides link to HPRD through its human protein databases (e.g. [http://www.ncbi.nlm.nih.gov/sites/entrez?db=gene Entrez Gene], [http://www.ncbi.nlm.nih.gov/RefSeq/ RefSeq protein]) pertaining to genes and proteins.

This resource depicts information on human protein functions including [[protein-proteinprotein–protein interactions]], [[post-translational modifications]], enzyme-substrate relationships and [[disease]] associations. Protein annotation information that is catalogued was derived through manual curation using published literature by expert biologists and through bioinformatics analyses of the protein sequence. The protein-proteinprotein–protein interaction and subcellular localization data from HPRD have been used to develop a human protein interaction network.<ref>{{cite journal | author = Gandhi, T.K.B. ''et| al.''title = Analysis of the human protein interactome and comparison with yeast, worm and fly interaction datasets. ''| journal = Nature Genetics'' | volume = 38| issue = 3| pages = 285–293 | doi=10.1038/ng1747 | pmid=16501559 | date=March 2006.| 3,s2cid = 1446423 285|display-293authors=etal}}</ref>

Highlights of HPRD as follows:

• * From 10,000 protein-proteinprotein–protein interactions (PPIs) annotated for 3,000 proteins in 2003, HPRD has grown to over 36,500 unique PPIs annotated for 25,000 proteins including 6,360 isoforms by the end of 2007.<ref>{{cite journal | author = Mathivanan, S. ''et| al.''year = 2006| title = An evaluation of human protein-proteinprotein–protein interaction data in the public ___domain. ''| journal = BMC Bioinformatics''. | volume = 2006. | issue = 7,| page = S19 | doi=10.1186/1471-2105-7-s5-s19|display-authors=etal | pmid=17254303 | pmc=1764475| doi-access = free}}</ref>

• * More than 50% of molecules annotated in HPRD have at least one PPI and 10% have more than 10 PPIs.

• * Experiments for PPIs are broadly grouped into three categories namely in vitro, in vivo and [[yeast two hybrid]] (Y2H). Sixty percent of PPIs annotated in HPRD are supported by a single experiment whereas 26% of them are found to have two of the three experimental methods annotated.

• More than 50% of molecules annotated in HPRD have at least one PPI and 10% have more than 10 PPIs.

• * HPRD contains 18,000 manually curated PTMs data belonging to 26 different types. [[Phosphorylation]] is the leading type of modification of protein contributing to 63% of PTM data annotated in HPRD. [[Glycosylation]], [[proteolytic cleavage]] and [[disulfide bridge]] events are the next leading contributors of PTM data.

• * HPRD data is available for download in tab delimited and [[XML]] file formats.<ref>{{cite journal | author = Mishra, G. ''et| al.''year = 2006| title = Human protein reference database--2006database—2006 update. ''| journal = Nucleic Acids Research''. 2006.| volume = 34,| 411issue = Database issue| pages = 411–414 | doi=10.1093/nar/gkj141|display-414authors=etal | pmid=16381900 | pmc=1347503}}</ref>

• Experiments for PPIs are broadly grouped into three categories namely in vitro, in vivo and [[yeast two hybrid]] (Y2H). Sixty percent of PPIs annotated in HPRD are supported by a single experiment whereas 26% of them are found to have two of the three experimental methods annotated.

• HPRD contains 18,000 manually curated PTMs data belonging to 26 different types. [[Phosphorylation]] is the leading type of modification of protein contributing to 63% of PTM data annotated in HPRD. [[Glycosylation]], [[proteolytic cleavage]] and [[disulfide bridge]] events are the next leading contributors of PTM data.

• HPRD data is available for download in tab delimited and [[XML]] file formats.<ref>Mishra, G. ''et al.'' Human protein reference database--2006 update. ''Nucleic Acids Research''. 2006. 34, 411-414</ref>

HPRD also integrates data from [[Human Proteinpedia]], a community portal for integrating human protein data. The data from HPRD can be freely accessed and used by academic users while commercial entities are required to obtain a license for use. Human Proteinpedia<ref>{{cite journal | author = Mathivanan, S. ''et| al.''year = 2008| title = Human Proteinpedia enables sharing of human protein data. ''| pmid = 18259167| journal = Nature Biotechnology''. 2008.| volume = 26,| issue = 2| pages = 164–167 | doi=10.1038/nbt0208-164|display-167authors=etal| hdl = 10261/60528| s2cid = 205265347| url = https://hal.inrae.fr/hal-02883742/file/nbt0208-164.pdf| hdl-access = free}}</ref> content is freely available for anyone to download and use.

==PhosphoMotif Finder==

PhosphoMotif Finder<ref>{{cite journal | author = Amanchy, R. ''et| al.''year = 2007| title = A compendium of curated phosphorylation-based substrate and binding motifs. ''| journal = Nature Biotechnology''. | volume = 2007. | issue = 25,| pages = 285–286 | doi=10.1038/nbt0307-285| pmid = 17344875| s2cid = 38824337|display-286authors=etal}}</ref> contains known kinase/phosphatase substrate as well as binding motifs that are curated from the published literature. It reports the PRESENCE of any literature-derived motif in the query sequence. PhosphoMotif Finder does NOT PREDICT any motifs in the query protein sequence using any algorithm or other computational strategies.

== Comparison of protein data ==

There are other databases that deal with human proteome (e.g. BioGRID, BIND, DIP, HPRD, IntAct, MINT, MIPS, PDZBase and Reactome). Each database has its own style of presenting the data. It is a difficult task for most investigators to compare the voluminous data from these databases in order to conclude strengths and weaknesses of each database. Mathivanan and colleagues <ref name="pmid17254303">{{cite journal |author=Mathivanan S, Periaswamy B, Gandhi TK, ''et al.'' |title=An evaluation of human protein-protein interaction data in the public ___domain |journal=[[BMC Bioinformatics]] |volume=7 Suppl 5 |issue= |pages=S19 |year=2006 |pmid=17254303 |pmc=1764475 |doi=10.1186/1471-2105-7-S5-S19 |urlname-list-style=http://www.biomedcentral.com/1471vanc |author2=Periaswamy B |author3=Gandhi TK |display-2105/7%20Suppl%205/S19authors=3 |last4=Kandasamy |first4=Kumaran |last5=Suresh |first5=Shubha |last6=Mohmood |first6=Riaz |last7=Ramachandra |first7=YL |last8=Pandey |first8=Akhilesh |issue=Suppl 5 |doi-access=free }}</ref> tried to address this issue while analyzing protein data by asking various questions. This analysis will help biologists to choose among these databases based on their needs.

== References ==

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==External links==

*http://www.humanproteinpedia.org {{Webarchive|url=https://web.archive.org/web/20070314135408/http://www.humanproteinpedia.org/ |date=2007-03-14 }}

*http://www.hprd.org {{Webarchive|url=https://web.archive.org/web/20060424071622/http://www.hprd.org/ |date=2006-04-24 }}

*http://www.hprd.org

[[Category:BioinformaticsProtein databases]]

[[Category:Online databases]]