Selector technique: Difference between revisions

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{{Unreferenced|date=October 2017}}{{Orphan|date=February 2009}}
The '''selector technique''' is a method to [[polymerase chain reaction|amplify]] and [[Multiplexing|multiplex]] genomic DNA.
 
== Process ==
Genomic [[DNA]] is digested with [[restriction enzymes]], circularized by [[hybridisation (molecular biology)|hybridisation]] to selectors and subsequently attached to a [[Plasmid|vector]] sequence by [[DNA ligase|ligation]]. The procedure results in circular DNA [[molecules]] with an included general [[Primer (molecular biology)|primer]] pair motif that can be used for amplification by [[Polymerase chain reaction|PCR]] or [[Rolling circle replication|RCA]].
 
==Selector construct==
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==Publications==
*[https://web.archive.org/web/20051127101236/http://nar.oxfordjournals.org/cgi/content/full/33/8/e71 Demonstration of the selector method]
*[https://archive.today/20130415163620/http://nar.oxfordjournals.org/cgi/content/full/33/8/e72 The PieceMaker software for designing selector experiments]
 
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