Selector technique: Difference between revisions

Content deleted Content added

Revision as of 04:05, 21 March 2020 edit Mbdfar (talk \| contribs) Autopatrolled, Extended confirmed users 16,671 edits de-orphaned ← Previous edit		Latest revision as of 00:26, 24 November 2022 edit undo BD2412 (talk \| contribs) Autopatrolled, Administrators 2,529,392 edits m →Process: Fixing links to disambiguation pages, replaced: PCR → PCR Tag: AWB
(2 intermediate revisions by 2 users not shown)
Line 3: == Process == Genomic [[DNA]] is digested with [[restriction enzymes]], circularized by [[hybridisation (molecular biology)\|hybridisation]] to selectors and subsequently attached to a [[Plasmid\|vector]] sequence by [[DNA ligase\|ligation]]. The procedure results in circular DNA [[molecules]] with an included general [[Primer (molecular biology)\|primer]] pair motif that can be used for amplification by [[Polymerase chain reaction\|PCR]] or [[Rolling circle replication\|RCA]]. ==Selector construct== Line 15: ==Publications== [https://web.archive.org/web/20051127101236/http://nar.oxfordjournals.org/cgi/content/full/33/8/e71 Demonstration of the selector method] [https://archive.today/20130415163620/http://nar.oxfordjournals.org/cgi/content/full/33/8/e72 The PieceMaker software for designing selector experiments] {{DEFAULTSORT:Selector-Technique}}