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[[File:Bio-layer interferometry without analyte binding.gif|thumb|Figure 1]]
[[File:Bio-layer interferometry with analyte binding.gif|thumb|Figure 2]]
[[File:Bio-layer interferometry wavelength shift due to analyte binding
'''Bio-layer interferometry''' ('''BLI''') is a [[label-free_quantification|label-free]] technology for measuring [[molecular interaction|biomolecular interactions]].<ref>{{cite journal |last1=Cooper |first1=Matthew |title=Current biosensor technologies in drug discovery. |journal=Drug Discovery World |date=May 7, 2006 |issue=Summer |pages=68–82 |url=https://www.ddw-online.com/drug-discovery/p97058-current-biosensor-technologies-in-drug-discoverysummer-06.html}}</ref><ref>{{cite journal |last1=Rich |first1=Rebecca L |last2=Myszka |first2=David G |title=Higher-throughput, label-free, real-time molecular interaction analysis. |journal=Analytical Biochemistry |date=1 February 2007 |volume=361 |issue=1 |pages=1–6 |doi=10.1016/j.ab.2006.10.040 |pmid=17145039 }}</ref> It is an optical analytical technique that analyzes the [[Interference (wave propagation)|interference]] pattern of white light reflected from two surfaces: a layer of immobilized [[protein]] on the biosensor tip, and an internal reference layer (Figure 1). Any change in the number of molecules bound to the biosensor tip causes a shift in the interference pattern that can be measured in real-time (Figures 1 and 2).
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