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The maximum separation between isogyres occurs when the slide is rotated exactly 45 degrees from one of the orientations where the isogyres come together. The point where the isogyres is most tightly curved represents the position of each of the two optic axes present for a biaxial mineral, and thus the maximum separation between the two curves is diagnostic of the angle between the two optic axes for the mineral. This angle is called the '''optic angle''' and often notated as '''"2V"'''. In some cases, knowing the optic angle can be a useful diagnostic tool to discriminate between two minerals which otherwise look very similar. In other cases, 2V varies with chemical composition in a known way for a given mineral, and its measured value can be used to estimate ratios between elements in the [[crystal structure]] – for example, Fe/Mg in [[olivine]]s. However, in these cases it becomes important to also be sure of the ''optic sign'' of the mineral (essentially, this tells you how the optic angle is orientated with respect to the whole [[optical indicatrix]] describing the refractive indices of the mineral in 3D). The optic sign and optic angle can be determined together by combining interference pattern microscopy with use of a [[sensitive tint plate]].
On either side of the "saddle" formed by the isogyres, birefringent rings of colour run concentrically around two eye like shapes called ''
A [[Interference colour chart|Michel-Levy Chart]] is often used in conjunction with the interference pattern to determine useful information that aids in the identification of minerals.
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