Plant transformation vector: Difference between revisions

Revision as of 04:25, 23 December 2023

Revision as of 03:48, 8 February 2024

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'''Plant transformation vectors''' are [[plasmid]]s that have been specifically designed to facilitate the generation of transgenic plants. The most commonly used plant transformation vectors are termed [[binary vectors|T-DNA binary vectors]] and are often replicated in both ''[[E. coli]]'', a common lab bacterium, and ''[[Agrobacterium tumefaciens]]'', a plant-virulent bacterium used to insert the recombinant (customized) DNA into plants.

Plant Transformation vectors contain three key elements;

* Plasmids Selection (creating a custom circular strand of DNA)

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==Plasmids replication==

[[Plasmids]] replicate to produce many plasmid molecules in each host bacterial cell. The number of copies of each plasmid in a bacterial cell is determined by the [[replication origin]], which is the position within the plasmid molecule where DNA replication is initiated. Most [[binary vectors]] have a higher number of plasmid copies when they replicate in ''[[E. coli]];'' however, the plasmid copy-number is usually lower when the plasmid is resident within ''[[Agrobacterium tumefaciens]]''.

Plasmids can also be replicated using the [[polymerase chain reaction]] (PCR).