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* '''Spacing between microelectrodes''': The spacing between microelectrodes (center-to-center) is typically larger than 300μm for sMEAs and 200 μm for glass MEAs. but can be less than 20μm in CMOS MEAs.
The reason for these differences is that sMEAs are fabricated using soft elastomeric materials such as [[Polydimethylsiloxane|PDMS]] as substrate and [[Micro-encapsulation|encapsulation]] which have a much higher coefficient of thermal expansion and lower Young's Modulus than rigid MEAs that are built on glass, plastic or silicon (CMOS) substrates. These properties make it more challenging to align and bond small features. In addition, the maximum [[Strain (mechanics)|strain]] that the electrodes can tolerate decreases for narrower electrodes, which is why the electrodes leads are often wide, thus limiting the number electrodes.<ref>{{cite journal |last1=Graudejus |first1=O. |last2=Jia |first2=Zheng |last3=Li |first3=Teng |last4=Wagner |first4=S. |title=Size-dependent rupture strain of elastically stretchable metal conductors |journal=Scripta Materialia |date=June 2012 |volume=66 |issue=11 |pages=919–922 |doi=10.1016/j.scriptamat.2012.02.034 |pmid=22773917 |pmc=3388513 }}</ref> sMEAs for in vitro applications are only available commercially from BioMedical Sustainable Elastic Electronic Devices.<ref>BMSEED. "Integrated Biomechanics, Imaging, & Electrophysiology." BMSEED, [https://www.bmseed.com www.bmseed.com]. Accessed 10 Nov. 2024.</ref>
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