Selector technique: Difference between revisions

Revision as of 14:44, 5 July 2007

Revision as of 14:45, 5 July 2007

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The '''selector technique''' allows multiplex amplification of arbitrary sets of genomic sequences. Genomic [[DNA]] is digested with restriction enzymes, circularized by [[hybridisation (molecular biology)|hybridisation]] to selectors and subsequently attached to a vector sequence by [[ligation]] [[ghjyt]]. The procedure results in circular [[DNA]] [[molecules]] with an included general primer pair motif that can be used for amplification by [[PCR]] or RCA.

====The selector construct====