Cellular microarray: Difference between revisions

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Revision as of 15:04, 15 February 2013

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A '''cellular microarray''', is a [[laboratory]] tool that allows for the [[multiplex (assay)|multiplex]] interrogation of living [[cell (biology)|cells]] on the surface of a solid support. The support, sometimes called a "chip", is spotted with varying materials, such as [[antibodies]], [[proteins]], or [[lipids]], which can interact with the cells, leading to their capture on specific spots. Combinations of different materials can be spotted in a given area, allowing not only cellular capture, when a specific interaction exists, but also the triggering of a cellular response, change in [[phenotype]], or detection of a response from the cell, such as a specific [[secretion|secreted]] factor. An example of cellular microarrays are [[PMHC Cellular Microarrays]].

There are a large number of types of cellular microarrays:

Cellular microarrays were developed by Daniel Chen, Yoav Soen, Dan Kraft, Patrick Brown and Mark Davis at [[Stanford University Medical Center]].

# [[Reverse transfection]] cell microarrays. David M. Sabatini's laboratory developed reverse-transfection cell microarrays at the [[Whitehead Institute]], publishing their work in 2001.

==References==

# [[PMHC Cellular microarraysMicroarrays]]. This type of microarray were developed by Daniel Chen, Yoav Soen, Dan Kraft, Patrick Brown and Mark Davis at [[Stanford University Medical Center]].

==References==

Chen DS, Davis MM (2006) Molecular and functional analysis using live cell microarrays. Curr Opin Chem Biol 10:28-34

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Soen Y, Chen DS, Stuge TB, Weber JS, Lee PP, et al. (2004) A novel cellular microarray identifies functional deficiences in tumor-specific T cell responses. J Clin Oncol 22:2510

Ziauddin J, Sabatini DM (2001) Microarrays of cells expressing defined cDNAs. Nature. 2001 May 3;411(6833):107-10. [http://www.ncbi.nlm.nih.gov/pubmed/11333987?dopt=Citation Pubmed link]

[[Category:Biotechnology]]