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'''Synthetic Genetic Array analysis (SGA)''' is a [[high-throughput screening|high-throughput]] technique for exploring [[synthetic lethality|synthetic lethal]] and synthetic sick [[genetic interactions]] ([[Synthetic lethality|SSL]]).<ref name="H. Tong 2001">{{cite pmid|11743205}}</ref> SGA allows for the systematic construction of double mutants using a combination of [[Recombinant DNA|recombinant genetic techniques]], mating and selection steps. Using SGA methodology a query gene deletion mutant can be crossed to an entire genome deletion set to identify any [[synthetic lethality|SSL]] interactions, yielding functional information of the query gene and the genes it interacts with. A large-scale application of SGA in which ~130 query genes were crossed to the set of ~5000 viable deletion mutants in yeast revealed a genetic network containing ~1000 genes and ~4000 SSL interactions.<ref>{{cite pmid|14764870}}</ref> The results of this study showed that genes with similar function tend to interact with one another and genes with similar patterns of genetic interactions often encode products that tend to work in the same pathway or complex. Synthetic Genetic Array analysis was initially developed using the model organism ''[[S.cerevisiae]]''. This method has since been extended to cover 30% of the ''S. cerevisiae'' genome.<ref>{{cite pmid|20093466}}</ref> Methodology has since been developed to allow SGA analysis in ''[[Schizosaccharomyces pombe|S.pombe]]''<ref>{{cite pmid|17893680}}</ref><ref>{{cite pmid|18931302}}</ref> and ''E.coli''.<ref>{{cite pmid|19160513}}</ref><ref>{{cite pmid|18677321}}</ref>
[[Image:Yeast colonies array 1536 format.jpg|thumb|300px|Arrayed yeast showing synthetic lethal interactions. Synthetic lethal interactions are those pairs of colonies with reduced or no growth.]]
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