DNA microarray: Difference between revisions

Revision as of 03:32, 25 March 2014

Revision as of 21:05, 3 April 2014

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{{Use dmy dates|date=December 2013}}

A '''[[DNA]] [[microarray]]''' (also commonly known as DNA chip or [[biochip]]) is a collection of microscopic DNA spots attached to a solid surface. Scientists use DNA microarrays to measure the [[Gene Expression|expression]] levels of large numbers of genes simultaneously or to [[genotype]] multiple regions of a genome. Each DNA spot contains [[Pico-|picomoles]] (10⁻¹² [[Mole (unit)|moles]]) of a specific DNA sequence, known as ''[[Hybridization probe|probe]]s'' (or ''reporters or [[oligonucleotide|oligo]]s''). These can be a short section of a [[gene]] or other DNA element that are used to [[Nucleic acid hybridization#Hybridization|hybridize]] a [[cDNA]] or cRNA (also called anti-sense RNA) <!--Agilent kit--> sample (called ''target'') under high-stringency conditions. Probe-target hybridization is usually detected and quantified by detection of [[fluorophore]]-, silver-, or [[chemiluminescence]]-labeled targets to determine relative abundance of nucleic acid sequences in the target.....

==The basic microarray==