Synthetic genetic array: Difference between revisions

Revision as of 20:33, 3 April 2016

Revision as of 16:59, 11 September 2016

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==Procedure==

Synthetic genetic array analysis is generally conducted using colony arrays on petriplates at standard densities (96, 384, 768, 1536). To perform a SGA analysis in ''S.cerevisae'', the query gene deletion is crossed systematically with a deletion mutant array (DMA) containing every viable knockout [[Open reading frame|ORF]] of the yeast genome (currently 4786 strains).<ref>{{cite web|title=Yeast Knockout Strains

|url=http://www.openbiosystems.com/GeneExpression/Yeast/YKO/|archiveurl=https://web.archive.org/web/20111119012937/http://www.openbiosystems.com/GeneExpression/Yeast/YKO/|archivedate=November 1719, 2011 |work=Open Biosystems}}</ref> The resulting [[diploids]] are then sporulated by transferring to a media containing reduced nitrogen. The [[haploid]] progeny are then put through a series of selection platings and incubations to select for double mutants. The double mutants are screened for SSL interactions visually or using imaging software by assessing the size of the resulting colonies.

[[Image:Pinning robot.jpg|thumb|280px|right|Replicating yeast colonies during SGA analysis using a pinning robot]]