Imaging particle analysis: Difference between revisions

Revision as of 09:03, 30 October 2016

Revision as of 10:49, 23 November 2016

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Static image acquisition is the most common form. Almost all microscopes can be easily adapted to accept a digital camera via a [[C mount]] adaptor. This type of set-up is often referred to as a [[digital microscope]], although many systems using that name are used only for displaying an image on a [[Video monitor#video display|monitor]].

The sample is prepared on a microscope slide which is placed on the [[Optical microscope#Stage|microscope stage]]. Once the sample has been focused on, then an image can be acquired and displayed on the monitor. If it is a [[digital camera]] or a [[frame grabber]] is present, the image can now be saved in digital format, and image processing algorithms can be used to isolate particles in the field of view and measure them.<ref>{{cite book|last=Russ|first=J.C.|title=Computer-Assisted Microscopy: The Measurement and Analysis of Images|year=1990|publisher=Springer US|isbn=978-1-4612-7868-9}}</ref><ref>{{cite book|lastauthor1=ShorteHazelwood, Kristin L.|firstauthor2=SOlenych, Scott G.L|author3=Griffin, John D.|author4=Cathcart, Judith (edA.)|author5=Davidson, Michael W.|editor1=Shorte, Spencer L.|editor2=Frischknecht, Friedrich|title=Imaging Cellular anand Molecular Biological Functions|year=2007|publisher=Springer|isbn=978-3-540-71330-2|pages=3–43|coauthors=Frischknecht, F. (ed.)|chapter=1.) Entering the Portal: Understanding the Digital Image Recorded Through a Microscope}}</ref>

In static image acquisition only one field of view image is captured at a time. If the user wishes to image other portions of the same sample on the slide, they can use the X-Y positioning hardware (typically composed of two [[linear stage]]s on the microscope to move to a different area of the slide. Care must be taken to insure that two images do not overlap so as not to count and measure the same particles more than once.