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The off-diagonal cells are used to indicate relationships between the elements. A marking of the cell indicates a directed link between two elements and can represent design relations or constraints between product components, communication between teams, information flow or precedence relations between activities. In one convention, reading across a row reveals the outputs that the element in that row provides to other elements, and scanning a column reveals the inputs that the element in that column receives from other elements. For example, in the DSM, the marking in row A and column C indicated a link from A to C (output from A, input to C). Alternatively, the rows and columns may be switched (without a change of meaning). Both conventions may be found in the literature.<ref name="DSMbook"/>
The cells along the diagonal are typically used to represent the system elements. However, the diagonal cells can be used for representing self-iterations (e.g., rework of a code that did not pass its unit testing). Self-iterations are required when a matrix element represents a block of activities/subsystems that may be further detailed, allowing hierarchical DSM structure.<ref>A. Karniel and Y. Reich, [
Two main categories of DSMs have been proposed: static and time-based.<ref name="DSMreview">T. Browning: [https://dx.doi.org/10.1109/17.946528 "Applying the Design Structure Matrix to System Decomposition and Integration Problems: A Review and New Directions."] In: ''IEEE Transactions on Engineering Management.'' 48(3):292-306, 2001.</ref>
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