Polymerase chain reaction: Difference between revisions

Revision as of 10:01, 2 November 2006

Revision as of 11:59, 2 November 2006

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{{redirect|PCR}}

'''Polymerase chain reaction''' ('''PCR''') is a [[molecular biology]] technique <ref>[http://www.si.edu/archives/ihd/videocatalog/9577.htm The history of PCR]: [[Smithsonian Institution]] Archives, Institutional History Division. Retrieved 24 June 2006.</ref> for [[enzyme|enzymatically]] [[DNA replication|replicating]] [[DNA]] without using a living [[organism]], such as ''[[E. coli]]'' or [[yeast]]. Like amplification using living organisms, the technique allows a small amount of DNA to be amplified exponentially. As PCR is an ''[[in vitro]]'' technique, it can be performed without restrictions on the form of DNA and it can be extensively modified to perform a wide array of [[Genetic engineering|genetic manipulations]].