Restriction fragment length polymorphism: Difference between revisions

Revision as of 23:38, 31 August 2018

Revision as of 18:51, 2 September 2018

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== Alternatives ==

The technique for RFLP analysis is, however, slow and cumbersome. It requires a large amount of sample DNA, and the combined process of probe labeling, DNA fragmentation, electrophoresis, blotting, hybridization, washing, and [[autoradiography]] could take up to a month to complete. A limited version of the RFLP method that used [[Oligomer restriction|oligonucleotide probes]] was reported in 1985.<ref name="SaikiScharf1985">{{cite journal|last1=Saiki|first1=R.|last2=Scharf|first2=S|last3=Faloona|first3=F|last4=Mullis|first4=K.|last5=Horn|first5=G.|last6=Erlich|first6=H.|last7=Arnheim|first7=N|title=Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia|journal=Science|volume=230|issue=4732|year=1985|pages=1350–1354|issn=0036-8075|doi=10.1126/science.2999980}}</ref> Fortunately, theThe results of the [[Human Genome Project]] have largely replaced the need for RFLP mapping, and the identification of many [[single-nucleotide polymorphism]]s (SNPs) in that project (as well as the direct identification of many disease genes and mutations) has replaced the need for RFLP disease linkage analysis (see [[SNP genotyping]]). The analysis of VNTR alleles continues, but is now usually performed by [[polymerase chain reaction]] (PCR) methods. For example, the standard [[National DNA database|protocols]] for [[DNA fingerprinting]] involve PCR analysis of [[CODIS|panels]] of more than a dozen VNTRs.

RFLP is still a technique used in marker assisted selection. Terminal restriction fragment length polymorphism (TRFLP or sometimes T-RFLP) is a [[molecular biology]] technique initially developed for characterizing bacterial communities in mixed-species samples. The technique has also been applied to other groups including soil fungi.