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# Data analysis tools
=== Mutant collection ===
The first step is to collect the mutants and create a mutant library either in solid or liquid media. Solid media could be better because it could save lots of time. At Early stage, mutant creation was done by homologous recombination method. We have an excellent mutant library for Saccharomyces cerevisiae, a well-studied model organism.
However, if you are trying for new, yeast model, you could have to either a genome sequencing and can predict the possible ORF by the good reference yeast genome(For example: with Saccharomyces cerevisiae). Consider a special case: If you don’t have a reference genome, you should go for transcriptome and genome analysis of that new model organism.<br />
==See also==
*[[Tetrad (genetics)]]
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