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Line 9: ===Absorbance=== Absorbance detection has been available in microplate readers for more than 3 decades and is used for assays such as [[ELISA]] assays, protein and nucleic acid quantification or enzyme activity assays<ref name="Mohamed-Bassem">{{cite journal\|last=Mohamed-Bassem\|first=A. Ashour\|date=12 February 1987\|title=Use of a 96-well microplate reader for measuring routine enzyme activities\|journal=[[Analytical Biochemistry]]\|volume= 166\|issue=2\|pages=353–360~~\|url=http://www.sciencedirect.com/science/article/pii/0003269787905859~~\|doi=10.1016/0003-2697(87)90585-9\|pmid=3434778}}</ref> (i.e. in the [[MTT assay]] for cell viability).<ref name="pmid6606682"> {{cite journal \| author = Mosmann, Tim

Plate reader: Difference between revisions