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* [https://github.com/evolvedmicrobe/dotplot dotplot] – R package to rapidly generate dot plots as either traditional or ggplot graphics.
* [http://sonnhammer.sbc.su.se/Dotter.html Dotter]<ref>{{Cite journal|last=Sonnhammer|first=E. L.|last2=Durbin|first2=R.|date=1995-12-29|title=A dot-matrix program with dynamic threshold control suited for genomic DNA and protein sequence analysis|journal=Gene|volume=167|issue=1–2|pages=GC1–10|issn=0378-1119|pmid=8566757|doi=10.1016/0378-1119(95)00714-8}}</ref> – Stand alone program to generate dot plots.
*[https://4virology.net/virology-ca-tools/jdotter/ JDotter]<ref>{{Cite journal|last=Brodie|first=Ryan|last2=Roper|first2=Rachel L.|last3=Upton|first3=Chris|date=2004-01-22|title=JDotter: a Java interface to multiple dotplots generated by dotter
* [https://github.com/molbio-dresden/flexidot Flexidot]<ref>{{Cite journal|last=Seibt|first=Kathrin M.|last2=Schmidt|first2=Thomas|last3=Heitkam|first3=Tony|title=FlexiDot: Highly customizable, ambiguity-aware dotplots for visual sequence analyses
* [http://cube.univie.ac.at/gepard Gepard]<ref>{{Cite journal|last=Krumsiek|first=Jan|last2=Arnold|first2=Roland|last3=Rattei|first3=Thomas|date=2007-04-15|title=Gepard: a rapid and sensitive tool for creating dotplots on genome scale
* [http://sourceforge.net/projects/genomdiff Genomdiff] – An open source Java dot plot program for viruses.
* [http://last.cbrc.jp/ LAST] for whole-genome "split-alignment"<ref>{{Cite journal |author=Frith MC. and Kawaguchi R. |title=Split-alignment of genomes finds orthologies more accurately. |journal=Genome Biol. |volume=16 |year=2015 | pages=106 |doi=10.1186/s13059-015-0670-9 |pmid=25994148 |pmc=4464727}}</ref>.
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