Single-chain variable fragment: Difference between revisions

Revision as of 06:00, 10 January 2020

Revision as of 11:27, 13 March 2020

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==Purification==

Single-chain variable fragments lack the constant [[Fc region]] found in complete antibody molecules, and, thus, the common binding sites (e.g., [[protein G]]) cannot be used to purify antibodies. These fragments can often be purified or immobilized using [[protein L]], since protein L interacts with the variable region of kappa light chains. More commonly, scientists incorporate a six histidine tag on the c-terminus of the scFv molecule and purify them using immobilized metal affinity chromatography (IMAC). For unknown reasons, someSome scFv can also be captured by [[protein A]] if they contain a human VH3 ___domain. <ref>https://pubmed.ncbi.nlm.nih.gov/9186782-staphylococcal-protein-a-binding-to-vh3-encoded-immunoglobulins/</ref>

==Bivalent and trivalent scFvs==