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===Nutrient/Serum Deprivation===
Elimination of [[Blood plasma|serum]] from the [[culture medium]] for about 24 hours results in the accumulation of cells at the transition between [[G0 phase|G<sub>0</sub>]] quiescence and early G1. This arrest is easily reversible through addition of serum or the deprived nutrient. Upon release, progression through the cell cycle is variable, as some cells remain quiescent while others proceed through the cell cycle at variable rates.<ref name=":1">{{Cite journal|last1=Davis|first1=Penny K|last2=Ho|first2=Alan|last3=Dowdy|first3=Steven F|date=June 2001|title=Biological Methods for Cell-Cycle Synchronization of Mammalian Cells|journal=BioTechniques|volume=30|issue=3|pages=1322–1331|doi=10.2144/01306rv01|pmid=11414226|doi-access=free}}</ref>
=== Contact Inhibition ===
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