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Experimentally, this value can be determined by determining [[diet (nutrition)|diet]]ary protein intake and then measuring [[nitrogen]] [[excretion]]. One formula for NPU is:{{cn|date=November 2017}}
:NPU = {0.16 × (24 hour protein intake in grams)} - {(24 hour urinary [[urea]] nitrogen) + 2} -
The [[PDCAAS|Protein Digestibility Corrected Amino Acid Score]] (PDCAAS) is a more modern rating for determining protein quality, and the current ranking standard used by the FDA.
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