Bio-layer interferometry: Difference between revisions

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Only molecules binding to or dissociating from the biosensor can shift the interference pattern and generate a response profile. Unbound molecules, changes in the [[refractive index]] of the surrounding medium, or changes in flow rate do not affect the interference pattern. This is a unique characteristic of bio-layer interferometry and extends its capability to perform in crude samples used in applications for protein-protein interactions,<ref>{{cite journal |last1=Fang |first1=Ye |title=Label-Free Cell-Based Assays with Optical Biosensors in Drug Discovery |journal=Assay and Drug Development Technologies |date=20 November 2006 |volume=4 |issue=5 |pages=583–595 |doi=10.1089/adt.2006.4.583 |pmid=17115929 }}</ref> quantitation, affinity,<ref>{{cite journal |last1=Fransson |first1=Johan |last2=Teplyakov |first2=Alexey |last3=Raghunathan |first3=Gopalan |last4=Chi |first4=Ellen |last5=Cordier |first5=Wendy |last6=Dinh |first6=Thai |last7=Feng |first7=Yiqing |last8=Giles-Komar |first8=Jill |last9=Gilliland |first9=Gary |last10=Lollo |first10=Bridget |last11=Malia |first11=Thomas J |last12=Nishioka |first12=Walter |last13=Obmolova |first13=Galina |last14=Zhao |first14=Shanrong |last15=Zhao |first15=Yonghong |last16=Swanson |first16=Ronald V |last17=Almagro |first17=Juan C |title=Human Framework Adaptation of a Mouse Anti-Human IL-13 Antibody |journal=Journal of Molecular Biology |date=30 April 2010 |volume=398 |issue=2 |pages=214–231 |doi=10.1016/j.jmb.2010.03.004 |pmid=20226193 }}</ref> and kinetics.<ref>{{cite journal |last1=Abdiche |first1=Yasmina |last2=Malashock |first2=Dan |last3=Pinkerton |first3=Alanna |last4=Pons |first4=Jaume |title=Determining kinetics and affinities of protein interactions using a parallel real-time label-free biosensor, the Octet |journal=Analytical Biochemistry |date=15 June 2008 |volume=377 |issue=2 |pages=209–217 |doi=10.1016/j.ab.2008.03.035 |pmid=18405656 }}</ref>

Bio-layer interferometry was invented by Dr. Hong Tan. He founded [[ForteBio]] in 2001, an instrument manufacturer based in [[FremontMenlo Park, California]]. FromIn 2011, ForteBio was acquired by Pall Corporation. After Danaher's acquisition of Pall, ForteBio remained under Pall until 2018. Between 2018 toand 2020, ForteBio was part of [[Molecular Devices]].<ref>{{cite web |title=Molecular Devices announces brand identity for its newly-formed biologics business unit |url=https://www.moleculardevices.com/news/molecular-devices-announces-brand-identity-its-newly-formed-biologics-business-unit |website=www.fortebio.com |accessdate=24 November 2018 |language=en}}</ref> In April 2020, ForteBio was acquired by [[Sartorius AG|Sartorius]].<ref>{{Cite web|title=News Releases {{!}} ForteBio|url=https://www.fortebio.com/news|access-date=2020-06-08|website=www.fortebio.com}}</ref>

Since 2019, an improved version of Bio-layer Interferometry technology has also been available from Gator Bio, a company founded by the same inventor. Gator Bio is located in Palo Alto, California.