Single particle analysis: Difference between revisions

Revision as of 12:28, 14 September 2021

Revision as of 12:35, 14 September 2021

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Single particle analysis can be done on both [[negative stain|negatively stained]] and vitreous ice-embedded [[Cryogenic transmission electron microscopy|CryoTEM]] samples. Single particle analysis methods are, in general, reliant on the sample being homogeneous, although techniques for dealing with [https://pubmed.ncbi.nlm.nih.gov/24089719/ conformational heterogeneity] are being developed.

Images (micrographs), in the past, were collected on film are digitizedtaken usingwith high-qualityan scannerselectron ormicroscope using built-in [[charge-coupled device|CCD]] detectors coupled to a phosphorescent layer. Now(in itthe ispast, commonthey towere useinstead directcollected electronon detectorsfilm toand collectdigitized imagesusing high-quality scanners). The image processing is carried out using specialized software [[Software tools for molecular microscopy|programs]], often run on multi-processor [[Computer cluster|computer clusters]]. Depending on the sample or the desired results, various steps of two- or three-dimensional processing can be done.

In addition, single particle analysis can also be performed in an individual particle mode using an ICP-MS unit.