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Citation bot (talk | contribs) Add: doi-access, s2cid, bibcode. Removed parameters. | Use this bot. Report bugs. | Suggested by Headbomb | Linked from Wikipedia:WikiProject_Academic_Journals/Journals_cited_by_Wikipedia/Sandbox | #UCB_webform_linked 1093/1125 |
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| pmid = 11743205
| pmc =
| bibcode = 2001Sci...294.2364T
| s2cid = 6505287
}}</ref> SGA allows for the systematic construction of double mutants using a combination of [[Recombinant DNA|recombinant genetic techniques]], mating and selection steps. Using SGA methodology a query gene deletion mutant can be crossed to an entire genome deletion set to identify any [[synthetic lethality|SSL]] interactions, yielding functional information of the query gene and the genes it interacts with. A large-scale application of SGA in which ~130 query genes were crossed to the set of ~5000 viable deletion mutants in yeast revealed a genetic network containing ~1000 genes and ~4000 SSL interactions.<ref>{{Cite journal
| last1 = Tong | first1 = A. H. Y.
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| pmid = 14764870
| pmc =
| bibcode = 2004Sci...303..808T
| s2cid = 11465508
}}</ref> The results of this study showed that genes with similar function tend to interact with one another and genes with similar patterns of genetic interactions often encode products that tend to work in the same pathway or complex. Synthetic Genetic Array analysis was initially developed using the model organism ''[[S. cerevisiae]]''. This method has since been extended to cover 30% of the ''S. cerevisiae'' genome.<ref>{{Cite journal
| last1 = Costanzo | first1 = M.
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| pmid = 20093466
| pmc = 5600254
| bibcode = 2010Sci...327..425C
}}</ref> Methodology has since been developed to allow SGA analysis in ''[[Schizosaccharomyces pombe|S.pombe]]''<ref>{{Cite journal
| last1 = Roguev | first1 = A.
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| pmid = 17893680
| pmc =
| s2cid = 21870145
}}</ref><ref>{{Cite journal
| last1 = Dixon | first1 = S. J.
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| pmid = 18931302
| pmc =2575475
| bibcode = 2008PNAS..10516653D
| doi-access = free
}}</ref> and ''E. coli''.<ref>{{Cite journal
| doi = 10.1038/nmeth.1240
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| pmc =
| s2cid = 205418664
}}</ref>
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==Background==
Synthetic genetic array analysis was initially developed by Tong et al.<ref name="H. Tong 2001"/> in 2001 and has since been used by many groups working in a wide range of biomedical fields. SGA utilizes the entire genome yeast knock-out set created by the yeast genome deletion project.<ref>{{cite web|url=http://www-sequence.stanford.edu/group/yeast_deletion_project/deletions3.html|title=''Saccharomyces'' Genome Deletion Project
==Procedure==
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