Angular resolution: Difference between revisions

Revision as of 03:42, 30 September 2021

Revision as of 18:22, 19 October 2021

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:<math>R=\frac{0.61\lambda}{\mathrm{NA}}\approx\frac{\lambda}{2\mathrm{NA}}</math>

The practical limit for <math>\theta</math> is about 70°. In a dry objective or condenser, this gives a maximum NA of 0.95. In a high-resolution [[oil immersion objective|oil immersion lens]], the maximum NA is typically 1.45, when using immersion oil with a refractive index of 1.52. Due to these limitations, the resolution limit of a light microscope using [[visible light]] is about 200&nbsp;[[nanometer|nm]]. Given that the shortest wavelength of visible light is [[Violet (color)|violet]] (<math>\lambda</math> ≈\approx 400&nbsp;\,\mathrm{nm}</math>),

:<math>R=\frac{1.22 \times 400\,\mbox{nm}}{1.45\ +\ 0.95}=203\,\mbox{nm}</math>