Bio-layer interferometry: Difference between revisions

Revision as of 08:03, 18 November 2021

Revision as of 08:03, 18 November 2021

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== Distinguishing Characteristics ==

[[File:Surface Plasmon Resonance (SPR).jpg|thumb|Overview schematic of Surface Plasmon Resonance|205x205px260x260px]]

BLI and SPR are both dominant technologies in the label-free instruments market.<ref name=":0" /> Despite sharing some similarities in concept, there are significant differences between the two techniques. Micro-fluidic SPR relies on a closed architecture to transport samples to a stationary sensor chip. BLI instead utilizes an open system, shaking multiple wells on a plate to transport the sensors to the samples without need for [[Microfluidics|micro-fluidics]].<ref name=":2">{{Cite journal|last1=Wallner|first1=Jakob|last2=Lhota|first2=Gabriele|last3=Jeschek|first3=Dominik|last4=Mader|first4=Alexander|last5=Vorauer-Uhl|first5=Karola|date=2013|title=Application of Bio-Layer Interferometry for the analysis of protein/liposome interactions|url=http://dx.doi.org/10.1016/j.jpba.2012.10.008|journal=Journal of Pharmaceutical and Biomedical Analysis|volume=72|pages=150–154|doi=10.1016/j.jpba.2012.10.008|pmid=23146240|issn=0731-7085}}</ref> Being a closed system, SPR's association and dissociation phases are limited by the technology's design. BLI's open plate design results in association and dissociation length limits determined by sample evaporation instead.<ref>{{Cite journal|last1=Abdiche|first1=Yasmina|last2=Malashock|first2=Dan|last3=Pinkerton|first3=Alanna|last4=Pons|first4=Jaume|date=2008|title=Determining kinetics and affinities of protein interactions using a parallel real-time label-free biosensor, the Octet|url=http://dx.doi.org/10.1016/j.ab.2008.03.035|journal=Analytical Biochemistry|volume=377|issue=2|pages=209–217|doi=10.1016/j.ab.2008.03.035|pmid=18405656|issn=0003-2697}}</ref> SPR is easily reproducible due to its continuous flow microfluidics. BLI's multi well plate design allows for extremely high throughput in one batch. [[Assay]] configuration in BLI can, in stable conditions, allow for recovery of samples. Assay configuration in SPR allows for higher sensitivity. As a result, BLI results are often compared to SPR results for validation.<ref>{{Cite journal|last1=Yang|first1=Danlin|last2=Singh|first2=Ajit|last3=Wu|first3=Helen|last4=Kroe-Barrett|first4=Rachel|date=2016|title=Comparison of biosensor platforms in the evaluation of high affinity antibody-antigen binding kinetics|url=http://dx.doi.org/10.1016/j.ab.2016.06.024|journal=Analytical Biochemistry|volume=508|pages=78–96|doi=10.1016/j.ab.2016.06.024|pmid=27365220|issn=0003-2697}}</ref>

== References ==

{{Reflist|2}}