Plant transformation vector: Difference between revisions

Revision as of 18:27, 10 May 2022

Revision as of 13:26, 21 May 2022

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==Plasmid selection==

When the bacteria with the desired, implanted gene are grown, they are made containing a selector. A selector is a way to isolate and distinguish the desired cells. A gene that makes the cells resistant to an antibiotic such as the antibiotics [[kanamycin]], [[ampicillin]], [[spectinomycin]] or [[tetracyclin]]<nowiki/>e is an easy selector to use. The desired cells (along with any other organisms growing within the culture) can be treated with an antibiotic allowing the desired cells to survive while other organisms cannot. The antibiotic gene is not usually transferred to the plant cell but remains within the bacterial cell.

==Plasmids replication==

[[Plasmids]] replicate to produce many plasmid molecules in each host bacterial cell. The number of copies of each plasmid in a bacterial cell is determined by the [[replication origin]]. This is the position within the plasmids molecule where DNA replication is initiated. Most [[binary vectors]] have a higher number of plasmidsplasmid copies when they replicate in ''[[E. coli]]'', the plasmid copy-number is usually less when the plasmid is resident within ''[[Agrobacterium tumefaciens]]''.

Plasmids can also be replicated in the [[polymerase chain reaction]] (PCR).