Transcriptomics technologies: Difference between revisions

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===Microarrays===
[[File:Summary of RNA Microarray.svg|thumb|500pxupright=2|''Summary of [[DNA microarray|DNA Microarrays]].'' Within the organisms, genes are transcribed and spliced (in eukaryotes) to produce mature mRNA transcripts (red). The mRNA is extracted from the organism and reverse transcriptase is used to copy the mRNA into stable ds-cDNA (blue). In microarrays, the ds-cDNA is fragmented and fluorescently labelled (orange). The labelled fragments bind to an ordered array of complementary oligonucleotides, and [[Fluorometer|measurement of fluorescent intensity]] across the array indicates the abundance of a predetermined set of sequences. These sequences are typically specifically chosen to report on genes of interest within the organism's genome.<ref name="Lowe_2017" />]]
 
====Principles and advances====