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: <math>{\partial^2 V_{ij}\over\partial x_i \, \partial y_j} = {-\gamma\over s_{ij}^2} {(x_j - x_i)}{(y_j-y_i)} </math>
The above is a direct outcome of one of the key underlying assumptions of ANM
The force constant of the system can be described by the [[Hessian matrix]] – (second partial derivative of potential ''V''):
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