Glycan array: Difference between revisions

Revision as of 14:39, 4 May 2023

Revision as of 14:39, 4 May 2023

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'''Glycan arrays''',<ref name="Carbohydrate Microarrays">{{cite journal|vauthors=Carroll GT, Wang D, Turro NJ, Koberstein JKJT|title=Photochemical Micropatterning of Carbohydrates on a Surface|journal=Langmuir|date=2006|volume=22|pages=2899–2905|doi=10.1021/la0531042}}</ref> like that offered by the [[Consortium for Functional Glycomics]] (CFG), [[National Center for Functional Glycomics]] (NCFG) and [http://www.zbiotech.com/ Z Biotech, LLC], contain carbohydrate compounds that can be screened with lectins, antibodies or cell receptors to define carbohydrate specificity and identify ligands. Glycan array screening works in much the same way as other microarray that is used for instance to study gene expression [[DNA microarrays]] or protein interaction [[Protein microarray]]s.

Glycan arrays are composed of various [[oligosaccharides]] and/or [[polysaccharides]] immobilised on a solid support in a spatially-defined arrangement.<ref name="Glycan arrays: recent advances and future challenges">{{cite journal|vauthors=Oyelaran O, Gildersleeve JC|title=Glycan arrays: recent advances and future challenges|journal=Curr Opin Chem Biol|date=Oct 2009|volume=13|issue=4|pages=406–413|doi=10.1016/j.cbpa.2009.06.021|pmid=19625207|pmc=2749919 }}</ref> This technology provides the means of studying glycan-protein interactions in a high-throughput environment. These natural or synthetic (see [[carbohydrate synthesis]]) glycans are then incubated with any glycan-binding protein such as [[lectin]]s, [[cell surface receptor]]s or possibly a whole organism such as a [[virus]]. Binding is quantified using fluorescence-based detection methods.