Single-chain variable fragment: Difference between revisions

Revision as of 11:40, 10 December 2021

Revision as of 20:52, 8 July 2023

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==Purification==

Single-chain variable fragments lack the constant [[Fc region]] found in complete antibody molecules, and, thus, the common binding sites (e.g., [[protein G]]) cannot be used to purify antibodies. These fragments can often be purified or immobilized using [[protein L]], since protein L interacts with the variable region of kappa light chains. More commonly, scientists incorporate a six histidine tag on the c-terminus of the scFv molecule and purify them using immobilized metal affinity chromatography (IMAC). Some scFv can also be captured by [[protein A]] if they contain a human VH3 ___domain. <ref>{{Cite journal |last=Vostakolaei |first=Mehdi Asghari |last2=Molavi |first2=Ommoleila |last3=Hejazi |first3=Mohammad Saeid |last4=Kordi |first4=Shirafkan |last5=Rahmati |first5=Saman |last6=Barzegari |first6=Abolfazl |last7=Abdolalizadeh |first7=Jalal |date=2019-09 |title=Isolation and characterization of a novel scFv antibody fragments specific for Hsp70 as a tumor biomarker |url=https://onlinelibrary.wiley.com/doi/10.1002/jcb.28732 |journal=Journal of Cellular Biochemistry |language=en |volume=120 |issue=9 |pages=14711–14724 |doi=10.1002/jcb.28732 |issn=0730-2312}}</ref><ref>{{Cite journal|url=https://pubmed.ncbi.nlm.nih.gov/9186782-staphylococcal-protein-a-binding-to-vh3-encoded-immunoglobulins/|pmid = 9186782|year = 1997|last1 = Potter|first1 = K. N.|last2 = Li|first2 = Y.|last3 = Pascual|first3 = V.|last4 = Capra|first4 = J. D.|title = Staphylococcal protein a binding to VH3 encoded immunoglobulins|journal = International Reviews of Immunology|volume = 14|issue = 4|pages = 291–308|doi = 10.3109/08830189709116521}}</ref><ref>{{Cite journal |last=Kordi |first=Shirafkan |last2=Rahmati-Yamchi |first2=Mohammad |last3=Asghari Vostakolaei |first3=Mehdi |last4=Barzegari |first4=Abolfazl |last5=Abdolalizadeh |first5=Jalal |date=2019-02-21 |title=Purification of a Novel Anti-VEGFR2 Single Chain Antibody Fragmentand Evaluation of Binding Affinity by Surface Plasmon Resonance |url=https://apb.tbzmed.ac.ir/Abstract/apb-22975 |journal=Advanced Pharmaceutical Bulletin |language=en |volume=9 |issue=1 |pages=64–69 |doi=10.15171/apb.2019.008 |issn=2228-5881 |pmc=PMC6468230 |pmid=31011559}}</ref>

==Bivalent and trivalent scFvs==

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* C6.5, a diabody targeting [[HER2/neu]]<ref name="Adams" /> found in some [[breast cancer]]s

* [[Brolucizumab]], a scFV binding to VEGF-A and used to treat wet age-related macular degeneration

* '''G6A''', a scFV binding to human Hsp70.1 and can be used to target this protein as a potential marker in vast cancers<ref>{{Cite journal |last=Vostakolaei |first=Mehdi Asghari |last2=Molavi |first2=Ommoleila |last3=Hejazi |first3=Mohammad Saeid |last4=Kordi |first4=Shirafkan |last5=Rahmati |first5=Saman |last6=Barzegari |first6=Abolfazl |last7=Abdolalizadeh |first7=Jalal |date=2019-09 |title=Isolation and characterization of a novel scFv antibody fragments specific for Hsp70 as a tumor biomarker |url=https://onlinelibrary.wiley.com/doi/10.1002/jcb.28732 |journal=Journal of Cellular Biochemistry |language=en |volume=120 |issue=9 |pages=14711–14724 |doi=10.1002/jcb.28732 |issn=0730-2312}}</ref>.

==References==