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Certain foodstuffs, such as [[Egg (food)|egg]]s or [[milk]], rate as 1 on an NPU chart.
Experimentally, this value can be determined by determining [[diet (nutrition)|diet]]ary protein intake and then measuring [[nitrogen]] [[excretion]]. One formula for apparent NPU is:<ref>{{cite journal |last1=Blackburn |first1=GL |last2=Bistrian |first2=BR |last3=Maini |first3=BS |last4=Schlamm |first4=HT |last5=Smith |first5=MF |title=Nutritional and metabolic assessment of the hospitalized patient
:NPU = {0.16 × (24 hour protein intake in grams)} - {(24 hour urinary [[urea]] nitrogen) + 2} - {0.1 × ([[ideal body weight]] in kilograms)} / {0.16 × (24 hour protein intake in grams)}
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