Expression vector: Difference between revisions

Revision as of 04:05, 13 August 2023

Revision as of 22:16, 18 August 2023

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===Plant===

Many plant expression vectors are based on the [[Ti plasmid]] of ''[[Agrobacterium tumefaciens]]''.<ref>{{cite journal |title=Techniques in plant molecular biology--progress and problems |vauthors=Walden R, Schell J |journal=European Journal of Biochemistry |year= 1990 |volume=192 |issue=3 |pages=563–76 |pmid= 2209611|doi=10.1111/j.1432-1033.1990.tb19262.x |doi-access=free }}</ref> In these expression vectors, DNA to be inserted into plant is cloned into the [[T-DNA Binary system|T-DNA]], a stretch of DNA flanked by a 25-bp direct repeat sequence at either end, and which can integrate into the plant genome. The T-DNA also contains the selectable marker. The ''Agrobacterium'' provides a mechanism for [[transformation (genetics)|transformation]], integration of into the plant genome, and the promoters for its ''vir'' genes may also be used for the cloned genes. Concerns over the transfer of bacterial or viral genetic material into the plant however have led to the development of vectors called intragenic vectors whereby functional equivalents of plant genome are used so that there is no transfer of genetic material from an alien species into the plant.<ref>{{cite book |url=https://books.google.com/books?id=mpc02lNJRs8C&pg=PT629 |title=Principles of Plant Genetics and Breeding|author= George Acquaah |date=16 August 2012|publisher= John Wiley & Sons Inc |isbn=9781118313695 }}</ref>

Plant viruses may be used as vectors since the ''Agrobacterium'' method does not work for all plants. Examples of plant virus used are the [[tobacco mosaic virus]] (TMV), [[potato virus X]], and [[cowpea mosaic virus]].<ref>{{cite journal |title= Use of viral vectors for vaccine production in plants |author1=M Carmen Cañizares |author2=Liz Nicholson |author3=George P Lomonossoff |journal=Immunology and Cell Biology |year=2005 |volume=83 |issue=3 |pages= 263–270 |doi=10.1111/j.1440-1711.2005.01339.x |pmid=15877604 |pmc=7165799 }}</ref> The protein may be expressed as a fusion to the coat protein of the virus and is displayed on the surface of assembled viral particles, or as an unfused protein that accumulates within the plant. Expression in plant using plant vectors is often constitutive,<ref>{{cite web |title=How Do You Make A Transgenic Plant? |url=http://cls.casa.colostate.edu/transgeniccrops/how.html |work=Department of Soil and Crop Sciences at Colorado State University }}</ref> and a commonly used constitutive promoter in plant expression vectors is the [[cauliflower mosaic virus]] (CaMV) 35S promoter.<ref>{{cite journal |author1=Fütterer J. |author2=Bonneville J. M. |author3=Hohn T |title=Cauliflower mosaic virus as a gene expression vector for plants |journal=Physiologia Plantarum