Structure validation: Difference between revisions

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==In cryo-EM==
Cyro-EM presents special challenges to model-builders as the observed electron density is frequently insufficient to resolve individual atoms, leading to a higher likelihood of errors. Geometry-based validation tools similar to those used in X-ray crystallography can be used to highlight implausible modeling choices and guide modeler toward more native-like structures. The CaBLAM method, which only uses C&alpha; atoms,<ref>{{cite web |title=CaBLAM Validation in Phenix |url=https://phenix-online.org/documentation/reference/cablam_validation.html |website=phenix-online.org}}</ref> is suitable for low-resolution structures from cyro-EM.<ref>{{cite journal |last1=Rohou |first1=Alexis |title=Improving cryo-EM structure validation |journal=Nature Methods |date=February 2021 |volume=18 |issue=2 |pages=130–131 |doi=10.1038/s41592-021-01062-1}}</ref>
 
== In SAXS ==