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In [[acute appendicitis]], total bilirubin can rise from 20.52 μmol/L to 143 μmol/L. In pregnant women, the total bilirubin level is low in all three trimesters.<ref name="Shivaraj 2009"/>
The measurement of bilirubin levels in the newborns is done through the use of bilimeter or transcutanoeus bilirubinometer instead of performing LFTs. When the total serum bilirubin increases over 95th percentile for age during the first week of life for high risk babies, it is known as [[hyperbilirubinemia]] of the newborn ([[neonatal jaundice]]) and requires [[light therapy]] to reduce the amount of bilirubin in the blood. Pathological jaundice in newborns should be suspected when the serum bilirubin level rises by more than 5 mg/dL per day, serum bilirubin more than the physiological range, clinical jaundice more than 2 weeks, and conjugated bilirubin (dark urine staining clothes). [[Hemolytic disease of the newborn|Haemolytic jaundice]] is the commonest cause of pathological jaundice. Those babies with Rh hemolytic disease, ABO incompatibility with the mother, [[Glucose-6-phosphate dehydrogenase]] (G-6-PD) deficiency and minor blood group incompatibility are at increased risk of getting haemolytic jaundice.<ref>{{cite journal|last1=Sana|first1=Ullah|last2=Khaista|first2=Rahman|last3=Mehdi|first3=Hedayati|title=Hyperbilirubinemia in Neonates: Types, Causes, Clinical Examinations, Preventive Measures and Treatments: A Narrative Review Article|journal=Iranian Journal of Public Health|date=May 2016|volume=45|issue=5|pages=558–568|pmid=27398328|pmc=4935699}}</ref>
===Alanine transaminase (ALT)===
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